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Soil

Soil analyses and biodegradation tests

We offer all the required analyses for a chemical or micro­bio­lo­gical assessment of solid samples and soils.1

In parti­cular the following micro­bio­lo­gical parameters can be examined:

Breathing activity and gas formation rate

In soil and waste samples diverse biolo­gical processes occur. They are especially based on conversion of organic carbon. Aerobic respi­ration tests and anaerobic fermen­tation tests can be used to evaluate waste samples. The German standard tests for waste deposits form the basis for this evaluation process.

 Respiration activity test (AT4) (in accordance with the Ordinance on Environmentally Compatible Storage of Waste)

  • The respi­ration activity (AT4) assesses the potential of test material to be biolo­gi­cally decom­posed under aerobic condi­tions. The oxygen consumption per unit of time (e.g 4 or 7 days) is deter­mined in the process and compared with corre­sponding blank tests. The respi­ration activity is an important parameter in the waste deposit ordinance, for the treatment and deposit of mecha­ni­cally and biolo­gi­cally pre-treated waste amongst other things.
  • We measure the AT4 in batch test in accordance with the regula­tions of “Ordinance on Environ­men­tally Compa­tible Storage of Waste from Human Settle­ments and on Biolo­gical Waste-Treatment Facilities” with the automatic measu­rement system OxiTop® (WTW).

Biogas potential test (Digester gas tests) (GB21)

  • Similar to breathing activity under aerobic condi­tions, the biogas potential test permits a statement on the ability and dynamic of a sample to be biolo­gi­cally decom­posed under anaerobic condi­tions. For this purpose the biolo­gical methane formation is automa­ti­cally analysed over a period of 21 days under anaerobic conditions.
  • This parameter is important for operators of biogas plants, for example. It provides the operator or substrate supplier with infor­mation on how much biogas can be produced from a defined substrate during fermen­tation process.
  • The fermen­tation test is conducted on the basis of German “DIN* 38414 Part 8” (German standard methods for the exami­nation of water, waste water and sludge). German standard tests on carrying out experi­ments can also be found in the “Ordinance on Environ­men­tally Compa­tible Storage of Waste from Human Settle­ments and on Biolo­gical Waste-Treatment Facilities“ and the VDI** Guideline 4630.

Compost maturity tests

Composts must be regularly examined within the scope of the German RAL quality assurance system (RAL-GZ 251). The compost maturity (degree of rotting) is regulated in the German Compost Ordinance and provides infor­mation on the maturing of the substrate during the course of the composting process. The more mature a compost is, the less oxygen it consumes in a defined period.

This measu­rement is carried out with the automatic measu­rement system OxiTop® (WTW). Measu­rement of the self-heating of the sample material in the Dewar container also provides infor­mation on the biolo­gical stability of the compost.

Biochemical oxygen demand – B.O.D. (DIN 38 409 H 51)

The bioche­mical oxygen demand for n days (BODn) is usually deter­mined during 5 days of incubation at 20°C. It represents the amount of oxygen needed for aerobic decom­posing processes of organic substances by micro­or­ga­nisms. The BOD therefore provides infor­mation on the biolo­gi­cally recyclable part of organic substances contained in a sample.

Diese Messung wird mit dem Messsystem OxiTop® (WTW) durchgeführt

Biodegradability of substances under defined conditions

Deter­mining the biode­gra­da­bility of lubri­cants and related products in accordance with DIN* 51828–2.

Inves­ti­gation of substances for easy biolo­gical decom­po­sition in accordance with the OECD guideline “Ready Biode­gra­da­bility” (301):

  • The methods are suitable for proving fast and complete biode­g­radtion under aerobic condi­tions. The testing substance becomes carbon dioxide, water and bio mass through microbial degra­dation process. The basis is the deter­mi­nation of unspe­cific parameters such as dissolved organic carbon, biolo­gical oxygen demand and carbon dioxide evolution.

Inves­ti­gation of substances for biode­gra­dation potential in accordance with OECD Guideline “Inherent Biode­gra­da­bility” (302):

  • The method is suitable for inves­ti­gating the inherent aerobic biode­gra­da­bility of organic substances even in the case of restricted ability to decompose of the test substances. It is parti­cu­larly useful for testing insoluble and/or volatile materials and based on the deter­mi­nation of the released organic carbon and the chemical oxygen demand.

We conduct individual testing possi­bi­lities in perco­lation pillars, circu­lation apparatus and individual experi­mental struc­tures going beyond the standard possi­bi­lities described.

1 partly in coope­ration with other laboratories

*DIN — German institute for Standar­dization
 **VDI — The Association of German Engineers