{"id":958,"date":"2024-04-03T16:05:19","date_gmt":"2024-04-03T14:05:19","guid":{"rendered":"https:\/\/micropro.de\/verfahren\/mpog-2\/"},"modified":"2025-09-23T10:39:03","modified_gmt":"2025-09-23T08:39:03","slug":"mpog-2","status":"publish","type":"page","link":"https:\/\/micropro.de\/en\/verfahren\/mpog-2\/","title":{"rendered":"MPOG\u00ae (Exploration)"},"content":{"rendered":"\n<p>The MPOG<sup>\u00ae<\/sup> technology, used to explore for oil and gas reser\u00advoirs, has been developed 50&nbsp;years ago by W. SCHWARZ und M.&nbsp;WAGNER.<\/p>\n\n\n\n<p>MPOG<sup>\u00ae<\/sup> \u2014 Microbial Prospecting for Oil and Gas is a surface explo\u00adration technology based on detection of signi\u00adficant popula\u00adtions of specific, hydrocarbon-degrading micro\u00ador\u00adga\u00adnisms in shallow soil samples (1\u20132&nbsp;m below surface). By appli\u00adcation of sophisti\u00adcated micro\u00adbio\u00adlo\u00adgical techniques total number and bioche\u00admical activity of various groups of methane\u2011, propane- and butane-oxidizing bacteria can reliably be deter\u00admined. Positive geo-microbial signals highlight regions with considerably increased bacterial cell number and activity \u2014 so called microbial anomalies \u2013 which can only develop on basis of additional hydro\u00adcarbon supply from micro-seepages verti\u00adcally above oil and gas bearing struc\u00adtures. Hence, the detection of such a microbial anomaly indicates a corre\u00adsponding hydro\u00adcarbon signature in the deeper underground.<\/p>\n\n\n\n<p>Appli\u00adcation of MPOG<sup>\u00ae<\/sup> enables the reliable diffe\u00adren\u00adtiation not only between hydro\u00adcarbon prospective and non-prospective areas but also between oil- and gas indica\u00adtions by identi\u00adfi\u00adcation of different groups of bacteria. Without an additional hydro\u00adcarbon supply from subsurface signi\u00adficant microbial popula\u00adtions cannot develop and merely background values will be&nbsp;recorded.<\/p>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-8 is-cropped lightbox wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-001-Probenahme-scaled.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1773\" height=\"2560\" data-id=\"865\" src=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-001-Probenahme-scaled.jpg\" alt=\"Geologen in Schutzkleidung entnehmen Bodenproben mit Handbohrer auf einem trockenen Feld, orangefarbene Erde sichtbar.\" class=\"wp-image-865\" srcset=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-001-Probenahme-scaled.jpg 1773w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-001-Probenahme-208x300.jpg 208w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-001-Probenahme-709x1024.jpg 709w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-001-Probenahme-768x1109.jpg 768w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-001-Probenahme-1064x1536.jpg 1064w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-001-Probenahme-1418x2048.jpg 1418w\" sizes=\"auto, (max-width: 1773px) 100vw, 1773px\"><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-002-scaled.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"2560\" height=\"1920\" data-id=\"868\" src=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-002-scaled.jpg\" alt=\"Mitarbeiter und Fahrzeuge bei geologischer Feldarbeit auf einer schlammigen Piste im Busch, Jakob Kretschmer steht vor einem Gel\u00e4ndewagen.\" class=\"wp-image-868\" srcset=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-002-scaled.jpg 2560w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-002-300x225.jpg 300w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-002-1024x768.jpg 1024w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-002-768x576.jpg 768w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-002-1536x1152.jpg 1536w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-002-2048x1536.jpg 2048w\" sizes=\"auto, (max-width: 2560px) 100vw, 2560px\"><\/a><\/figure>\n<\/figure>\n\n\n\n<div style=\"height:130px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group left-padding-box\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading special\">Microbial Prospecting for Oil and Gas (MPOG\u00ae) offers the following advantages:<\/h2>\n\n\n\n<ul class=\"wp-block-list pt-0\">\n<li>Reliable diffe\u00adren\u00adtiation between hydro\u00adcarbon prospective and non-prospective areas<\/li>\n\n\n\n<li>Reliable, affordable and rapid&nbsp;results<\/li>\n\n\n\n<li>Infor\u00admation about the infill of struc\u00adtures (oil,&nbsp;gas)<\/li>\n\n\n\n<li>Cost-reduction for explo\u00adration by exclusion of non-prospecting areas<\/li>\n\n\n\n<li>Appli\u00adcable in all regions, onshore and&nbsp;offshore<\/li>\n\n\n\n<li>Effective also in difficult terrain<\/li>\n\n\n\n<li>Repro\u00addu\u00adcible results also on complex geolo\u00adgical&nbsp;structures<\/li>\n\n\n\n<li>Repeated and comple\u00admentary measu\u00adre\u00adments possible (after 1&nbsp;year)<\/li>\n\n\n\n<li>Fast, easy and environmentally-sound sampling technology<\/li>\n\n\n\n<li>Most efficient in combi\u00adnation with other explo\u00adration surveys (seismic acquisition)<\/li>\n\n\n\n<li>No \u201chalo\u201d effects<\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div style=\"height:130px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-pb-accordion-item c-accordion__item js-accordion-item no-js\" data-initially-open=\"false\" data-click-to-close=\"true\" data-auto-close=\"true\" data-scroll=\"true\" data-scroll-offset=\"100\"><h3 id=\"at-9580\" class=\"c-accordion__title js-accordion-controller\" role=\"button\">Method<\/h3><div id=\"ac-9580\" class=\"c-accordion__content\">\n<h3 class=\"wp-block-heading special\">Basis oft Microbial Prospecting for Oil and&nbsp;Gas<\/h3>\n\n\n\n<p>The basis of MPOG<sup>\u00ae<\/sup> is that oil or gas fields emit a conti\u00adnuous stream of light hydro\u00adcarbon gases to the earth\u2019s surface known as macro- and&nbsp;micro-seepages.<\/p>\n\n\n\n<p>In all shallow soil and sediment layers a variety of specia\u00adlized micro-organisms (hydro\u00adcarbon oxidizing bacteria) exists, able to utilize extremely low concen\u00adtra\u00adtions of short-chain hydro\u00adcarbons (10<sup>-6<\/sup> %vol\/vol). Based on a conti\u00adnuous hydro\u00adcarbon supply active popula\u00adtions of hydro\u00adcarbon degrading bacteria can develop. This long term supply is almost exclu\u00adsively found at micro-seepages above hydro\u00adcarbon bearing struc\u00adtures. Under these condi\u00adtions detec\u00adtable popula\u00adtions of bacteria develop with signi\u00adfi\u00adcantly increased cell numbers and a high bioche\u00admical&nbsp;activity.<\/p>\n\n\n\n<p>For oil and gas explo\u00adration two well distin\u00adgu\u00adis\u00adhable groups of bacteria are&nbsp;relevant:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Hydro\u00adcarbon oxidizing bacteria<\/li>\n\n\n\n<li>Methane oxidizing bacteria (Methy\u00adlo\u00adtrophic)<\/li>\n<\/ul>\n\n\n\n<p>Due to high specia\u00adlization of methane-oxidizing bacteria, the MPOG<sup>\u00ae<\/sup> method allows methane-oxidizers to be isolated from all other bacteria and bioche\u00admi\u00adcally analyzed. A signi\u00adfi\u00adcantly increased activity and cell number of methane oxidizing bacteria represents an indication for methane occur\u00adrence in soil&nbsp;samples.<\/p>\n\n\n\n<p>Another bioche\u00admical group of micro\u00ador\u00adga\u00adnisms uses short-chained hydro\u00adcarbons (C<sub>2<\/sub> \u2014 C<sub>8<\/sub>) as energy source. These micro-organisms are not able to metabolize methane. Short alkanes namely ethane, propane and butane can be utilized by a large variety of bacteria. Detection of active bacteria which oxidize n\u2011alkanes with chain lengths of 2 to 8 carbon atoms without any adapt\u00adation period indicates the existence of oil signa\u00adtures in the area&nbsp;investigated.<\/p>\n\n\n\n<p>On the basis of a complex micro\u00adbio\u00adlo\u00adgical test scheme to determine bacterial count and bioche\u00admical activity of specific bacteria, an oil and gas indication factor will be recorded for each soil or sediment sample. Maps with identified microbial hydro\u00adcarbon anomalies show position and extent of hydro\u00adcarbon indications.<\/p>\n\n\n\n<p>The higher the calcu\u00adlated measure units (cell numbers and activity), the more intense the hydro\u00adcarbon supplies to bacteria and the greater the proba\u00adbility of finding hydro\u00adcarbon accumu\u00adla\u00adtions at these&nbsp;positions.<\/p>\n\n\n\n<p>MPOG<sup>\u00ae<\/sup> data can easily be integrated into existing explo\u00adration data sets (e.g. seismic) or used for pre-selection of parti\u00adcu\u00adlarly interesting areas for subse\u00adquent, cost-intensive explo\u00adration techniques (wild-cat exploration).griert werden oder zur Vorauswahl besonders inter\u00ades\u00adsanter Fl\u00e4chen f\u00fcr nachfol\u00adgende, kosten\u00adin\u00adten\u00adsivere Verfahren genutzt werden (Wild-cat exploration).<\/p>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-2 is-cropped lightbox wp-block-gallery-2 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-003-Bitumen.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1024\" data-id=\"862\" src=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-003-Bitumen.jpg\" alt=\"Bitumenhaltiger Boden mit \u00d6lfilm und Schimmer in einer feuchten Senke zwischen Grasb\u00fcscheln.\" class=\"wp-image-862\" srcset=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-003-Bitumen.jpg 768w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-003-Bitumen-225x300.jpg 225w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\"><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-004-Covering-hole-1.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1536\" height=\"864\" data-id=\"859\" src=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-004-Covering-hole-1.jpg\" alt=\"Arbeiter in Schutzkleidung bedeckt mit einer Hacke eine Bodenprobe-Stelle im Grasland.\" class=\"wp-image-859\" srcset=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-004-Covering-hole-1.jpg 1536w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-004-Covering-hole-1-300x169.jpg 300w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-004-Covering-hole-1-1024x576.jpg 1024w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-004-Covering-hole-1-768x432.jpg 768w\" sizes=\"auto, (max-width: 1536px) 100vw, 1536px\"><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-005-Drilling-1.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1536\" height=\"864\" data-id=\"856\" src=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-005-Drilling-1.jpg\" alt=\"Techniker mit Schutzhelm f\u00fchrt eine manuelle Bohrung im Erdreich mit Bohrstange durch.\" class=\"wp-image-856\" srcset=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-005-Drilling-1.jpg 1536w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-005-Drilling-1-300x169.jpg 300w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-005-Drilling-1-1024x576.jpg 1024w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-005-Drilling-1-768x432.jpg 768w\" sizes=\"auto, (max-width: 1536px) 100vw, 1536px\"><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-006-GC-sampling-1.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1536\" height=\"864\" data-id=\"853\" src=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-006-GC-sampling-1.jpg\" alt=\"Arbeiter f\u00fcllt entnommene Bodenprobe aus dem Bohrer in ein Probengef\u00e4\u00df ab.\" class=\"wp-image-853\" srcset=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-006-GC-sampling-1.jpg 1536w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-006-GC-sampling-1-300x169.jpg 300w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-006-GC-sampling-1-1024x576.jpg 1024w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-006-GC-sampling-1-768x432.jpg 768w\" sizes=\"auto, (max-width: 1536px) 100vw, 1536px\"><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-007-GC-sampling-8.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1536\" height=\"864\" data-id=\"850\" src=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-007-GC-sampling-8.jpg\" alt=\"Mitarbeiter zeigt ein gef\u00fclltes Isotech-Probenglas, w\u00e4hrend Kollegen im Hintergrund weitere Proben vorbereiten.\" class=\"wp-image-850\" srcset=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-007-GC-sampling-8.jpg 1536w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-007-GC-sampling-8-300x169.jpg 300w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-007-GC-sampling-8-1024x576.jpg 1024w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-007-GC-sampling-8-768x432.jpg 768w\" sizes=\"auto, (max-width: 1536px) 100vw, 1536px\"><\/a><\/figure>\n<\/figure>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-pb-accordion-item c-accordion__item js-accordion-item no-js\" data-initially-open=\"false\" data-click-to-close=\"true\" data-auto-close=\"true\" data-scroll=\"true\" data-scroll-offset=\"100\"><h3 id=\"at-9581\" class=\"c-accordion__title js-accordion-controller\" role=\"button\"><strong>Application<\/strong><\/h3><div id=\"ac-9581\" class=\"c-accordion__content\">\n<h3 class=\"wp-block-heading special\"><strong>Application of Microbial Prospecting for Oil and&nbsp;Gas<\/strong><\/h3>\n\n\n\n<p>Basically no geolo\u00adgical or seismic data is required to carry out a geo-microbial explo\u00adration survey. In areas which have not yet been inves\u00adti\u00adgated by geophysics, the MPOG<sup>\u00ae<\/sup> technique can be applied as a wild-cat explo\u00adration tool, in order to identify hydro\u00adcarbon signa\u00adtures in <strong>green field areas<\/strong>. In this way subse\u00adquent geolo\u00adgical and seismic inves\u00adti\u00adga\u00adtions can thus be focused on hydrocarbon-prospective areas. Measu\u00adrement points are laid over the area to be inves\u00adti\u00adgated in the form of a map grid. The stationary interval is from 500&nbsp;m to a maximum of 2,000&nbsp;m depending on the expected reservoir size.<\/p>\n\n\n\n<p>In those regions where structure data of the sub-surface already exists, the stationary interval can be reduced to 250 \u2014 500&nbsp;m, in order to generate a more detailed picture of the hydro\u00adcarbon distri\u00adbution. Likewise, this approach does not require any knowledge of the position of geologic struc\u00adtures. As a result therefore, the seismic structure maps and microbial anomalies, which have been recorded independently from one another, can be compared and&nbsp;contrasted.<\/p>\n\n\n\n<p>A further appli\u00adcation of microbial prospecting is <strong>reservoir charac\u00adte\u00adrization<\/strong>. In this case the distance between two measuring points should not exceed 200&nbsp;m. The area of inves\u00adti\u00adgation is restricted to an already known structure. This method is parti\u00adcu\u00adlarly suited for evaluation of the reservoir dimen\u00adsions and altera\u00adtions of the oil-water contact due to long-term oil production processes.Additional appli\u00adca\u00adtions of MPOG<sup>\u00ae<\/sup> are frequent measu\u00adre\u00adments within a safety monitoring program at under\u00adground gas storages or along&nbsp;pipelines.<\/p>\n\n\n\n<h3 class=\"wp-block-heading special\">Sampling procedure<\/h3>\n\n\n\n<p>Onshore, soil samples are taken in the field at pre-defined positions using an environ\u00admen\u00adtally friendly hand auger set at a depth of 1 \u2014 2&nbsp;m. At each measu\u00adrement point about 200&nbsp;g of soil shall be packed in airtight sterile sampling bags. A tempo\u00adr\u00adarily storage at 4 \u2014 10\u00b0C and trans\u00adpor\u00adtation to the laboratory should not exceed a period of 14&nbsp;days.<\/p>\n\n\n\n<p>Offshore, seabed core samples are taken by means of a vibro-coring system or grab-sampler approx. 0.5 \u2014 2&nbsp;m below sediment surface. Sampling material for MPOG<sup>\u00ae<\/sup> analyses can easily be sub-sampled from cores on board a&nbsp;vessel.<\/p>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-2 is-cropped lightbox wp-block-gallery-3 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-008-Isojars.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"960\" height=\"1280\" data-id=\"847\" src=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-008-Isojars.jpg\" alt=\"Geologen bereiten IsoJar-Probengef\u00e4\u00dfe f\u00fcr die Gaschromatographie im Feld unter einer Plane vor.\" class=\"wp-image-847\" srcset=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-008-Isojars.jpg 960w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-008-Isojars-225x300.jpg 225w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-008-Isojars-768x1024.jpg 768w\" sizes=\"auto, (max-width: 960px) 100vw, 960px\"><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-009-MPOG-sampling-4.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1536\" height=\"864\" data-id=\"844\" src=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-009-MPOG-sampling-4.jpg\" alt=\"Nahaufnahme: Bodenprobe wird mit einem Werkzeug in ein beschriftetes Probenglas gef\u00fcllt, Utensilien liegen auf einer Plane.\" class=\"wp-image-844\" srcset=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-009-MPOG-sampling-4.jpg 1536w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-009-MPOG-sampling-4-300x169.jpg 300w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-009-MPOG-sampling-4-1024x576.jpg 1024w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-009-MPOG-sampling-4-768x432.jpg 768w\" sizes=\"auto, (max-width: 1536px) 100vw, 1536px\"><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-010-Wet-area.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1600\" height=\"1200\" data-id=\"841\" src=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-010-Wet-area.jpg\" alt=\"Team in Schutzkleidung durchquert sumpfiges Gebiet mit kniehohem Wasser w\u00e4hrend geologischer Feldarbeit.\" class=\"wp-image-841\" srcset=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-010-Wet-area.jpg 1600w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-010-Wet-area-300x225.jpg 300w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-010-Wet-area-1024x768.jpg 1024w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-010-Wet-area-768x576.jpg 768w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-010-Wet-area-1536x1152.jpg 1536w\" sizes=\"auto, (max-width: 1600px) 100vw, 1600px\"><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-011-Sample-picture.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1536\" height=\"864\" data-id=\"838\" src=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-011-Sample-picture.jpg\" alt=\"Feldtechnikerin in orangefarbener Schutzkleidung und Helm dokumentiert Bodenproben mit einem Tablet, Probenbeh\u00e4lter und T\u00fctchen liegen auf einer Unterlage im Gras.\" class=\"wp-image-838\" srcset=\"https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-011-Sample-picture.jpg 1536w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-011-Sample-picture-300x169.jpg 300w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-011-Sample-picture-1024x576.jpg 1024w, https:\/\/micropro.de\/wp-content\/uploads\/2025\/09\/Exploration-011-Sample-picture-768x432.jpg 768w\" sizes=\"auto, (max-width: 1536px) 100vw, 1536px\"><\/a><\/figure>\n<\/figure>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-pb-accordion-item c-accordion__item js-accordion-item no-js\" data-initially-open=\"false\" data-click-to-close=\"true\" data-auto-close=\"true\" data-scroll=\"true\" data-scroll-offset=\"100\"><h3 id=\"at-9582\" class=\"c-accordion__title js-accordion-controller\" role=\"button\">Case histories<\/h3><div id=\"ac-9582\" class=\"c-accordion__content\">\n<h3 class=\"wp-block-heading special\"><strong>Microbial Prospecting for Oil and Gas \u2013 case&nbsp;histories<\/strong><\/h3>\n\n\n\n<p>The following case studies compare and contrast results of geo-microbial surface prospecting (MPOG<sup>\u00ae<\/sup>) with seismic and geolo\u00adgical data. At the time of micro\u00adbio\u00adlo\u00adgical inves\u00adti\u00adgation, the authors did not have knowledge of any of the data on geolo\u00adgical struc\u00adtures of the inves\u00adti\u00adgation areas, apart from already existing wells. The case studies were selected on basis of their exemplary character and different geolo\u00adgical&nbsp;setting.<\/p>\n\n\n\n<p>The map on the right shows the positions of surveyed areas both onshore and offshore in Central Europe.<\/p>\n\n\n\n<p>The following colors repre\u00adsenting MPOG<sup>\u00ae <\/sup>data were used for the maps discussed here.<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-table fallbeispiele-table\"><table><thead><tr><th class=\"has-text-align-left\" data-align=\"left\">Erd\u00f6l<\/th><th class=\"has-text-align-left\" data-align=\"left\">Erdgas<\/th><th class=\"has-text-align-left\" data-align=\"left\">Kategorie<\/th><\/tr><\/thead><tbody><tr><td class=\"has-text-align-left\" data-align=\"left\"><\/td><td class=\"has-text-align-left\" data-align=\"left\"><\/td><td class=\"has-text-align-left\" data-align=\"left\">Background<\/td><\/tr><tr><td class=\"has-text-align-left\" data-align=\"left\"><\/td><td class=\"has-text-align-left\" data-align=\"left\"><\/td><td class=\"has-text-align-left\" data-align=\"left\">Incon\u00adclusive zone<\/td><\/tr><tr><td class=\"has-text-align-left\" data-align=\"left\"><\/td><td class=\"has-text-align-left\" data-align=\"left\"><\/td><td class=\"has-text-align-left\" data-align=\"left\">anomaly B<\/td><\/tr><tr><td class=\"has-text-align-left\" data-align=\"left\"><\/td><td class=\"has-text-align-left\" data-align=\"left\"><\/td><td class=\"has-text-align-left\" data-align=\"left\">anomaly A<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>A division of recorded microbial activities into four levels is mainly based on inves\u00adti\u00adga\u00adtions in regions with ecolo\u00adgical condi\u00adtions like in central Europe and the North Sea. Under extreme ecolo\u00adgical condi\u00adtions (e.g. in desert regions) the average of measured activities can decline. Nevert\u00adheless, a reliable identi\u00adfi\u00adcation of positive microbial anomalies is guaranteed because of decreased background level.<\/p>\n\n\n\n<p>The background level, which is mainly of biogenic origin, does not indicate any signi\u00adficant fluctua\u00adtions. The additional hydro\u00adcarbon supply from oil and gas fields (e.g. from depths of ca. 2,000&nbsp;m), which is confirmed by isotope analyses (delta <sup>13<\/sup>C<sub>1<\/sub> = \u201130 to \u201140 (\u2030) PDB), causes signi\u00adficant microbial anomalies.<\/p>\n\n\n\n<p>By separately identi\u00adfying methane and hydro\u00adcarbon oxidizing bacteria, it is possible to diffe\u00adren\u00adtiate between oil and gas reser\u00advoirs, and oil reser\u00advoirs with a gas cap. Oil fields without a free gas cap have either no or small methane indica\u00adtions, but do have signi\u00adficant oil indica\u00adtions. Oil fields with a free gas cap create measurable methane anomalies and increased oil indica\u00adtions. Gas fields in the North Sea, which contain beside methane also aliphatic hydro\u00adcarbons (ca. 15%) show oil indica\u00adtions as well as signi\u00adficant methane indica\u00adtions. The intensity of these oil indica\u00adtions however is normally below the indication level for oil&nbsp;fields.<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-pb-accordion-item c-accordion__item js-accordion-item no-js\" data-initially-open=\"false\" data-click-to-close=\"true\" data-auto-close=\"true\" data-scroll=\"true\" data-scroll-offset=\"100\"><h3 id=\"at-9583\" class=\"c-accordion__title js-accordion-controller\" role=\"button\">MPOG \u2014&nbsp;Publications<\/h3><div id=\"ac-9583\" class=\"c-accordion__content\">\n<ul class=\"wp-block-list\">\n<li><strong>Baum, M., M. Schmitt, M. Wagner, C. Westerlage (2008)<\/strong><br>&nbsp;Geoche\u00admical and Micro\u00adbio\u00adlo\u00adgical Surface Inves\u00adti\u00adgation in Northern Germany Indicates Interesting Hydro\u00adcarbon Potential: Oil Gas European Magazine 1\/2008; 10\u201314<\/li>\n\n\n\n<li><strong>Trappe, H.; Wagner, M.; Bleschert, K.; Piske, J.: (2004)<\/strong><br>&nbsp;Verkn\u00fcpfung von mikro\u00adbieller Oberfl\u00e4\u00adchen\u00adpro\u00adspektion mit seismi\u00adschen Attri\u00adbuten am Beispiel einer osteu\u00adro\u00adp\u00e4i\u00adschen \u00d6llagerst\u00e4tte;Vortrag auf der Fr\u00fchjahrs\u00adtagung der DGMK, Celle, Germany<\/li>\n\n\n\n<li><strong>Wagner, M., et.al.: (2003)<\/strong><br>&nbsp;MPOG- Microbial Prospection of Oil and Gas; Field Examples and their Geolo\u00adgical Background, Lecture on the Inter\u00adna\u00adtional Symposium \u201cRecent Trends in surface geoche\u00admical Prospecting and Risk Reduction for hydro\u00adcarbon Explo\u00adration and Develo\u00adpment\u201d; Hyderabad, India<\/li>\n\n\n\n<li><strong>Wagner, M., M.Wagner, J. Piske, R. Smit (2002)<\/strong><br>&nbsp;Case Histories of Microbial Prospection for Oil and Gas Onshore and Offshore in north-west Europe<br>&nbsp;AAPG-SEG Special Publi\u00adcation \u2014 Appli\u00adcation of Surface Explo\u00adration Methods to Explo\u00adration, Field Develo\u00adpment and Production, edited by D. Schumacher and L.&nbsp;Le-Schack<\/li>\n\n\n\n<li><strong>Wagner, M., M. Wagner, (1999)<\/strong><br>&nbsp;Microbial Prospection for Oil and Gas \u2014 Case Histories on- and offshore:<br>&nbsp;Confe\u00adrence Ravenna, Italy, 2, 1061\u20131067.<\/li>\n\n\n\n<li><strong>Wagner, M., H.J. Rasch, J. Piske, and B. Ziran, (1998)<\/strong><br>&nbsp;Mikro\u00adbielle Prospektion auf Erd\u00f6l und Erdgas in Ostdeutschland:<br>&nbsp;Geolo\u00adgi\u00adsches Jahrbuch, 149, 287\u2013301.<\/li>\n\n\n\n<li><strong>Wagner, M., M. Wagner, H.J. Rasch, J. Piske, and M. Baum, (1998)<\/strong><br>&nbsp;MPOG \u2014 Microbial prospection for oil and gas. Field examples and their geolo\u00adgical background:<br>&nbsp;Confe\u00adrence Cracov, Poland, AO-05, 118\u2013121.<\/li>\n\n\n\n<li><strong>Baum, M.G., M. Wagner et.a. (1997)<\/strong><br>&nbsp;Appli\u00adcation of Surface Prospecting Methods in the Dutch North Sea<br>&nbsp;Petroleum Geoscience 3, 171\u2013181<\/li>\n\n\n\n<li><strong>Wagner, M. (1966)<\/strong><br>&nbsp;Ergeb\u00adnisse der mikro\u00adbio\u00adlo\u00adgi\u00adschen Prospektion im K\u00fcsten\u00adbe\u00adreich der Ostsee<br>&nbsp;In Vortr\u00e4ge des Inter\u00adna\u00adtio\u00adnalen Symposium \u201eErd\u00f6l\u00admi\u00adkro\u00adbio\u00adlogie\u201c, Brno 1964 (Malek &amp; Schwartz, Ed.), Akademie Verlag Berlin<\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The MPOG\u00ae technology, used to explore for oil and gas reser\u00advoirs, has been developed 50&nbsp;years ago by W. SCHWARZ und M.&nbsp;WAGNER. MPOG\u00ae \u2014 Microbial Prospecting for Oil and Gas is a surface explo\u00adration technology based on detection of signi\u00adficant popula\u00adtions of specific, hydrocarbon-degrading micro\u00ador\u00adga\u00adnisms in shallow soil samples (1\u20132&nbsp;m below surface). By appli\u00adcation of&nbsp;sophisticated&nbsp;[\u2026]<\/p>\n","protected":false},"author":1,"featured_media":315,"parent":949,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-full-width-image.php","meta":{"wp_typography_post_enhancements_disabled":false,"_hupa_show_title":true,"_hupa_custom_title":"","_hupa_title_css":"","_hupa_select_menu":1,"_hupa_select_sidebar":1,"_hupa_show_social_media":false,"_hupa_select_social_type":0,"_hupa_select_social_color":0,"_hupa_social_media_css":"","_hupa_select_handy_menu":1,"_hupa_select_top_area":1,"_hupa_show_bottom_footer":true,"_hupa_show_top_footer":false,"_hupa_show_widgets_footer":false,"_hupa_sticky_widgets_footer":1,"_hupa_select_header":0,"_hupa_select_container":0,"_hupa_top_area_container":0,"_hupa_main_container":0,"_hupa_select_footer":0,"_hupa_show_menu":true,"_hupa_show_custom_url":false,"_hupa_beitragsbild_url":"","footnotes":""},"class_list":["post-958","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>MPOG\u00ae (Exploration) - MicroPro GmbH Microbiological Laboratories Gommern<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/micropro.de\/en\/verfahren\/mpog-2\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"MPOG\u00ae (Exploration) - MicroPro GmbH Microbiological Laboratories Gommern\" \/>\n<meta property=\"og:description\" content=\"The MPOG\u00ae technology, used to explore for oil and gas reser\u00advoirs, has been developed 50&nbsp;years ago by W. 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